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University of Nevada

5 ARTICLES PUBLISHED IN JoVE

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Behavior

Task Interruption and Resumption Paradigm for Testing the Activation and Pursuit of an Abstract Thinking Goal
Jae-Eun Namkoong 1, Marlone D. Henderson 2
1Managerial Sciences Department, University of Nevada, 2Department of Psychology, University of Texas

This protocol was designed to test the activation and pursuit of cognitive goals (e.g., an abstract thinking goal) using the task interruption and resumption paradigm. The protocol is suitable for cognitive goals that are automatically pursued once activated, as the distraction procedure prevents goal pursuit during the interruption period.

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JoVE Journal

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
David A. Clark 1,2, Donovan Kohler 1, America Mathis 1, Eryn Slankster 1, Samipya Kafle 1, Seth R. Odell 1,2, Dennis Mathew 1,2
1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada

This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.

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Neuroscience

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
Dante J. Heredia 1, Grant W. Hennig 2, Thomas W. Gould 1
1Department of Physiology and Cell Biology, School of Medicine, University of Nevada, 2Department of Pharmacology, Larner College of Medicine, University of Vermont

Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.

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Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release
Berfin Sucu 1, Ayşe Bayraktar 1,2, Hatice Duman 1, Ayşenur Arslan 1, Merve Kaplan 1, Melda Karyelioğlu 1, Eda Ntelitze 1, Taner Taştekin 1, Seray Yetkin 1, Melih Ertürk 2, Steven A. Frese 3,4, Bethany M. Henrick 4,5, Hacı Mehmet Kayili 6, Bekir Salih 7, Sercan Karav 1
1Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, 2Uluova Dairy Farm, 3Department of Nutrition, University of Nevada, 4Department of Food Science and Technology, University of Nebraska, 5Evolve BioSystems Inc., 6Department of Biomedical Engineering, Karabuk University, 7Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.

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Biochemistry

Formulation and Characterization of Bioactive Agent Containing Nanodisks
Kyle Lethcoe 1, Colin A. Fox 1, Isabel Moh 1, Matthew Swackhamer 1, Michael Karo 1, Ryan Lockhart 1, Robert O. Ryan 1
1Department of Biochemistry and Molecular Biology, University of Nevada

Here, we describe the production and characterization of bioactive agents containing nanodisks. Amphotericin B nanodisks are taken as an example to describe the protocol in a stepwise manner.

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