Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

1 ARTICLES PUBLISHED IN JoVE

Biology

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Arianna Psichas^*1, Gwen Tolhurst^*1, Cheryl A. Brighton¹, Fiona M. Gribble¹, Frank Reimann¹

¹Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

This protocol describes the isolation and culture of mixed murine small intestinal cells. These primary intestinal cultures enable the investigation of signaling pathways underlying gut peptide secretion using a number of techniques.