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Szent István University

3 ARTICLES PUBLISHED IN JoVE

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Biology

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
Zoran Marinović 1, Jelena Lujić 1, Eszter Kása 1, Zsolt Csenki 1, Béla Urbányi 1, Ákos Horváth 1
1Department of Aquaculture, Szent István University

The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.

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Developmental Biology

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
Juan G. Herranz-Jusdado 1, Eszter Kása 2, Tímea Kollár 2, Víctor Gallego 1, David S. Peñaranda 1, Christoffer Rozenfeld 1, Luz Pérez 1, Ákos Horváth 2, Juan F. Asturiano 1
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

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Environment

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
Zsolt Csenki 1, Ákos Horváth 1, Illés Bock 1, Edina Garai 1, Flóra Kerekes 1, Erna Vásárhelyi 1, Balázs Kovács 1, Béla Urbányi 1, Ferenc Mueller 2, Katalin Bakos 1
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).

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