McGill University and Génome Québec Innovation Centre

3 ARTICLES PUBLISHED IN JoVE

Biology

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Cecile M. Perrault¹, Mohammad A. Qasaimeh¹, David Juncker¹

¹Department of Biomedical Engineering, McGill University

In this video we present the microfluidic probe¹ (MFP). We explain in detail how to assemble the MFP, mount it atop an inverted microscope, and align it relative to the substrate surface, and finally show how to use it to process a substrate surface immersed in a buffer solution.

Chemistry

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Huiyan Li¹, Robert Popp¹, Bjorn Frohlich¹, Michael X. Chen², Christoph H. Borchers^1,3,4,5

¹University of Victoria-Genome BC Proteomics Centre, ²Jewish General Hospital, McGill University, ³Dept of Biochemistry and Microbiology, University of Victoria, ⁴Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, ⁵Gerald Bronfman Department of Oncology, Jewish General Hospital

A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.

Bioengineering

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Huiyan Li^1,2, Sebastien Bergeron³, Heidi Larkin^1,2,3, David Juncker^1,2

¹McGill University and Génome Québec Innovation Centre, ²Biomedical Engineering Department, McGill University, ³Parallex BioAssays Inc.

We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.