JoVE Logo

Sign In

Aix Marseille Univ

3 ARTICLES PUBLISHED IN JoVE

image

Biology

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
Vincent Rouger 1,2,3, Nicolas Bertaux 4,5,6, Tomasz Trombik 1,2,3, Sébastien Mailfert 1,2,3, Cyrille Billaudeau 1,2,3, Didier Marguet 1,2,3, Arnauld Sergé 1,2,3
1Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, 3Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, 4École Centrale Marseille, Technopôle de Château-Gombert, 5Institut Fresnel, Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University

Multiple-Target Tracing is a homemade algorithm developed for tracking individually labeled molecules within the plasma membrane of living cells. Efficiently detecting, estimating and tracing molecules over time at high-density provide a user-friendly, comprehensive tool to investigate nanoscale membrane dynamics.

image

Bioengineering

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
Martine Pithioux *1,2, Flavy Roseren *1,2, Christian Jalain 4, Franck launay 1,3, Philippe Charpiot 5, Patrick chabrand 1,2, Sandrine Roffino 1, Edouard Lamy 1,6
1CNRS, ISM, Inst Movement Sci, Aix Marseille Univ, 2Sainte-Marguerite Hospital, Institute for Locomotion, Department of Orthopaedics and Traumatology, APHM, 3Sainte-Marguerite Hospital, Institute for Locomotion, Department of Peadiatric Orthopaedics, APHM, 4Ecole centrale de Marseille, 5Faculté de Pharmacie, Laboratoire de Biochimie, 6Service Central de la Qualité et de l'Information Pharmaceutiques, APHM

This study describes a reproducible and detailed protocol using a newly developed external fixator for distraction osteogenesis (DO) in a femoral rat model which permits physiological weight-bearing by the animal after removal of the external fixator.

image

Biochemistry

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Sebastien Mailfert *1, Karolina Wojtowicz *2, Sophie Brustlein *1, Ewa Blaszczak 3, Nicolas Bertaux 4, Marcin Łukaszewicz 2, Didier Marguet 1, Tomasz Trombik 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved