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University of Liege

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Methodology for Sputum Induction and Laboratory Processing
Julien Guiot *1, Sophie Demarche *1,2, Monique Henket 1, Virginie Paulus 1, Sophie Graff 1, Florence Schleich 1, Jean-Louis Corhay 1, Renaud Louis 1, Catherine Moermans 1
1Department of Respiratory Medicine, CHU Liege, GIGA I3 Research Group, University of Liege, 2Department of Clinical Pharmacy, CIRM (Center for Interdisciplinary Research on Medicines), University of Liege

Here we describe the technique of sputum induction. This protocol also explains the sputum processing to perform a differential cell count and to collect sputum supernatant and cells for further analysis.

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Medicine

Nerve Stimulator-guided Injection of Autologous Stem Cells Near the Equine Left Recurrent Laryngeal Nerve
Charlotte Sandersen 1, Justine Ceusters 1, Alexia Fourez 1, Irene Tosi 1, Helene Graide 1, Jean-Philippe Lejeune 1,2, Didier Serteyn 1,2
1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 2Mont-le-Soie Equine Research Centre

Here, we present a protocol to inject autologous muscle-derived stem cells in the proximity to the recurrent laryngeal nerve with the help of an electrical nerve stimulator. This novel technique may become useful for the treatment of equine recurrent laryngeal neuropathy.

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