Kings College London

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Caroline Haupt^*1, Tommy Hofmann^*1, Sabine Wittig^*1, Susann Kostmann¹, Argyris Politis², Carla Schmidt¹

¹Interdisciplinary research center HALOmem, Martin Luther University Halle-Wittenberg, ²Department of Chemistry, Kings College London

The architecture of protein complexes is essential for their function. Combining various mass spectrometric techniques proved powerful to study their assembly. We provide protocols for chemical cross-linking and native mass spectrometry and show how these complementary techniques help to elucidate the architecture of multi-subunit protein assemblies.

Research

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Zainab Ahdash¹, Andy M. Lau¹, Chloe Martens¹, Argyris Politis¹

¹Department of Chemistry, King's College London

Mass spectrometry (MS) has emerged as an important tool for the investigation of structure and dynamics of macromolecular assemblies. Here, we integrate MS-based approaches to interrogate protein complex formation and ligand binding.