JoVE Logo

Sign In

Université de Namur

1 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
Jean-Michel Vandeweerd 1, Fanny Hontoir 1, Alexis De Knoop 1, Kathleen De Swert 2, Charles Nicaise 2
1URVI, NARILIS, Université de Namur, 2URPhyM, NARILIS, Université de Namur

Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved