High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic DevicesCéline Braïni 1, Angelo Mottolese 1, Ivan Ferrante 1, Sylvain Monnier 2, Catherine Villard 1
1Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique, Université PSL, CNRS, 2UMR 144 Institut Curie, Université PSL, CNRS
Volume is an important parameter regarding physiological and pathological characteristics of cells. We describe a fluorescent exclusion method allowing full-field measurement of in vitro neuronal volume with sub-micrometric axial resolution required for the analysis of neurites and dynamic structures implied in neuronal growth.
