Beijing Academy of Science and Technology

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Xiaojing Lin^1,2, Tingbao Zhao³, Wenhui Xiong¹, Shaonan Wen⁴, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology, Indiana University School of Medicine, ²Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA, General Hospital of Jinan Military Region, ³Department of Orthopedics, Shandong Cancer Hospital, Shandong University, ⁴Department of Neurobiology, Institute of Basic Medical Sciences, Academy Military Medical Sciences

We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy in vivo.

Medicine

Spinal Cord Lateral Hemisection and Asymmetric Behavioral Assessments in Adult Rats

Xiao-Jing Lin^1,2,3, Shaonan Wen^2,6, Ling-Xiao Deng³, Heqiao Dai³, Xiaolong Du³, Chen Chen^3,4, Melissa J. Walker^3,4, Ting-Bao Zhao^1,5, Xiao-Ming Xu³

¹Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA, 960th Hospital, Joint Logistics Support Force of PLA, ²Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences, ³Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman Campbell Brain and Spine, Indiana University School of Medicine, ⁴Program in Medical Neuroscience, Paul and Carole Stark Neurosciences Research Institute, Indiana University School of Medicine, ⁵Department of Orthopedics, the Third Affiliated Hospital, Shandong University, ⁶Beijing Computing Center, Beijing Academy of Science and Technology

Here we describe surgical procedures to produce a reliable spinal cord lateral hemisection (HX) at the 9^th thoracic level in adult rats and neurobehavioral assessments designed for detecting asymmetric deficits after such a unilateral injury.