University of Hawaii at Manoa

2 ARTICLES PUBLISHED IN JoVE

Environment

The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide

Melissa Anne Johnson^1,2, Robert Hollingsworth², Samuel Fortna^2,3, Luis F. Aristizábal⁴, Nicholas C. Manoukis²

¹Oak Ridge Institute for Science and Education, ²Daniel K. Inouye US Pacific Basin Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, ³College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, ⁴Independent Consultant on CBB Management

Comprehensive monitoring of coffee berry borer and host plant dynamics is essential for aggregating landscape-level data to improve management of this invasive pest. Here, we present a protocol for scientific monitoring of coffee berry borer movement, infestation, mortality, coffee plant phenology, weather, and farm management via a mobile electronic data recording application.

Environment

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

Weiting Jiao^1,2, Tingting Shen², Luyao Wang¹, Lei Zhu^1,3, Qing X. Li⁴, Chen Wang³, Hongping Chen³, Rimao Hua¹, Xiangwei Wu¹

¹Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, ²State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, ³Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, ⁴Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa

The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens.