Universidad Católica de Valencia San Vicente Mártir

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Miguel Martí^*1, Belén Frígols^*1, Angel Serrano-Aroca¹

¹Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir

We present a protocol for the antimicrobial characterization of advanced materials. Here, the antimicrobial activity on material surfaces is measured by two methods that complement each other: one is based on the agar disk diffusion test, and the other is a standard procedure based on the ISO 22196:2007 norm.

Biology

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Elisa Oltra^1,2, Alejandro Caicedo³

¹School of Medicine and Dentistry, Universidad Católica de Valencia San Vicente Mártir, ²Unidad Mixta CIPF-UCV, Centro de Investigación Príncipe Felipe, ³Department of Medicine, University of Miami

The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.