UPV-EHU, CIBERDEM

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Irati Romero-Garmendia^*1, Amaia Jauregi-Miguel^*1, Izortze Santin², Jose Ramón Bilbao¹, Ainara Castellanos-Rubio¹

¹Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, ²Endocrinology and Diabetes Research Group, Biocruces Health Research Institute, UPV-EHU, CIBERDEM

Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.