Borough of Manhattan Community College/CUNY

2 ARTICLES PUBLISHED IN JoVE

Biology

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Jun Liang¹, Sheng Xiong^2,3, Cathy Savage-Dunn^2,3

¹Department of Science, Borough of Manhattan Community College, City Universtiy of New York (CUNY), ²Department of Biology, Queens College, The City University of New York (CUNY), ³Biochemistry Program, The Graduate Center, Queens College, The City University of New York (CUNY)

We demonstrate how to use the RNAi feeding technique to knock down target genes and score body size phenotype in C. elegans. This method could be used for a large scale screen to identify potential genetic components of interest, such as those involved in body size regulation by DBL-1/TGF-β signaling.

Biology

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Jun Liang¹, Aijo De Castro¹, Lizette Flores¹

¹Department of Science, Borough of Manhattan Community College/CUNY

This protocol demonstrates how to track a protein nuclear translocation under heat stress by using a green fluorescence protein (GFP) fusion protein as a marker and 4',6-diamidino-2-phenylindole (DAPI) staining. The DAPI staining protocol is fast and preserves the GFP and protein subcellular localization signals.