Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff PerfusionMiroslava Stastna 1,2,3, Amandine Thomas 1, Juliana Germano 1, Somayeh Pourpirali 1, Jennifer E. Van Eyk 1,2, Roberta A. Gottlieb 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences
Here we present a protocol to perform polysome profiling on the isolated perfused mouse heart. We describe methods for heart perfusion, polysome profiling, and analysis of the polysome fractions with respect to mRNAs, miRNAs, and the polysome proteome.