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Aintree University Hospital NHS Foundation Trust

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
Andrea M. Collins 1,2, Jamie Rylance 3, Daniel G. Wootton 4, Angela D. Wright 3,5, Adam K. A. Wright 1,3, Duncan G. Fullerton 3,6, Stephen B. Gordon 3
1Biomedical Research Centre in Microbial Diseases, National Institute for Health Research, 2Respiratory Infection Group, Royal Liverpool and Broadgreen University Hospital Trust, 3Respiratory Infection Group, Liverpool School of Tropical Medicine, 4Institute of Infection and Global Health, University of Liverpool, 5Comprehensive Local Research Network, Royal Liverpool and Broadgreen University Hospital Trust, 6Department of Respiratory Research, University Hospital Aintree

We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.

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Immunology and Infection

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Kyle Taruc 1, Charles Yin 1, Daniel G. Wootton 2,3, Bryan Heit 1
1Department of Microbiology and Immunology and the Center for Human Immunology, University of Western Ontario, 2Institute of Infection and Global Health, University of Liverpool, 3Department of Respiratory Research, Aintree University Hospital NHS Foundation Trust

Efferocytosis, the phagocytic removal of apoptotic cells, is required to maintain homeostasis and is facilitated by receptors and signaling pathways that allow for the recognition, engulfment, and internalization of apoptotic cells. Herein, we present a fluorescence microscopy protocol for the quantification of efferocytosis and the activity of efferocytic signaling pathways.

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