Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene EditingNa Tang 1,2, Yaoyao Zhang 1, Miriam Pedrera 1, Pengxiang Chang 1, Susan Baigent 1, Katy Moffat 1, Zhiqiang Shen 2, Venugopal Nair 1, Yongxiu Yao 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases
Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.