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Tampere University Hospital

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
Hanna Luukinen 1, Milka Marjut Hammarén 1, Leena-Maija Vanha-aho 1, Mataleena Parikka 1,2
1Faculty of Medicine and Life Sciences, University of Tampere, 2Oral and Maxillofacial Unit, Tampere University Hospital

Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.

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Immunology and Infection

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
Henna Myllymäki *1, Mirja Niskanen *1, Kaisa Oksanen 1,2, Mika Rämet 1,3,4,5
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu

Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.

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Medicine

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
Ashok Aspatwar 1, Milka Marjut Hammaren 1, Mataleena Parikka 1,2, Seppo Parkkila 1,3
1Faculty of Medicine and Health Technology, Tampere University, 2Oral and Maxillofacial Unit, Tampere University Hospital, 3Fimlab Ltd, Tampere University Hospital

Zebrafish embryos are used for evaluating the toxicity of chemical compounds. They develop externally and are sensitive to chemicals, allowing detection of subtle phenotypic changes. The experiment only requires a small amount of compound, which is directly added to the plate containing embryos, making the testing system efficient and cost-effective.

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Medicine

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
Maria Vähätupa 1,2, Niina Jääskeläinen 2, Marc Cerrada-Gimenez 2, Rubina Thapa 2, Tero Järvinen 1, Giedrius Kalesnykas 2, Hannele Uusitalo-Järvinen 1,3
1Faculty of Medicine and Health Technology, Tampere University & Tampere University Hospital, 2Experimentica Ltd, 3Eye Centre, Tampere University Hospital

Oxygen-induced retinopathy (OIR) can be used to model ischemic retinal diseases such as retinopathy of prematurity and proliferative diabetic retinopathy and to serve as a model for proof-of-concept studies in evaluating antiangiogenic drugs for neovascular diseases. OIR induces robust and reproducible neovascularization in the retina that can be quantified.

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Medicine

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia
Rüdiger Schultz 1,2, Tiina Peromaa 3, Heikki Lukkarinen 2, Varpu Elenius 2
1Allergy Centre, Tampere University Hospital, 2Department of Pediatrics, Turku University Hospital, 3Turku Bioscience Center, University of Turku and Åbo Akademi University

High-speed video microscopy analysis is a relatively easy-to-perform, fast, cost-effective, and, in experienced hands, a considerably reliable tool for first-line diagnostics of primary ciliary dyskinesia, which should be available in every center involved in diagnostics and the treatment of severe lung diseases.

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