Canadian National Transplant Research Program

2 ARTICLES PUBLISHED IN JoVE

Medicine

Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism

Sanaz Hatami¹, Christopher W. White¹, Martin Ondrus¹, Xiao Qi¹, Max Buchko¹, Sayed Himmat¹, Lily Lin², Katie Cameron³, David Nobes⁴, Hyun-Joong Chung⁵, Jayan Nagendran^1,6, Darren H. Freed^1,3,6,7

¹Department of Surgery, Faculty of Medicine, University of Alberta, ²Department of Pediatrics, Faculty of Medicine, University of Alberta, ³Department of Biomedical Engineering, Faculty of Medicine, University of Alberta, ⁴Department of Mechanical Engineering, Faculty of Engineering, University of Alberta, ⁵Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, ⁶Canadian National Transplant Research Program, ⁷Department of Physiology, Faculty of Medicine, University of Alberta

Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.

Medicine

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

Keir A. Forgie^1,2, Nicholas Fialka³, Max Buchko^1,2, Sayed Himmat⁴, Sanaz Hatami⁴, Xiao Qi⁴, Xiuhua Wang⁴, Katie-Marie Buswell⁵, Ryan Edgar⁵, Darren H. Freed^1,2,6,7, Jayan Nagendran^1,2,6,7

¹Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, ²Mazankowski Alberta Heart Institute, ³Faculty of Medicine and Dentistry, University of Alberta, ⁴Department of Surgery, Faculty of Medicine, University of Alberta, ⁵Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, ⁶Alberta Transplant Institute, ⁷Canadian Donation and Transplantation Research Program

This paper describes a porcine model of negative pressure ventilation ex situ lung perfusion, including procurement, attachment, and management on the custom-made platform. Focus is made on anesthetic and surgical techniques, as well as troubleshooting.