Friedrich Alexander University Erlangen-Nuremberg

2 ARTICLES PUBLISHED IN JoVE

Engineering

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

André Gemeinhardt¹, Matthew P. McDonald^1,2, Katharina König^1,3, Michael Aigner⁴, Andreas Mackensen⁴, Vahid Sandoghdar^1,3

¹Max Planck Institute for the Science of Light (MPL), ²Area of Scientific Learning, Milligan College, ³Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, ⁴Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.

Medicine

Polytetrafluoroethylene (PTFE) as a Suture Material in Tendon Surgery

Elias Polykandriotis^1,2, Marcus Himmler³, Shirin Mansouri², Florian Ruppe⁴, Jasmin Grüner¹, Lars Bräeuer⁵, Dirk W. Schubert³, Raymund E. Horch¹

¹Department of Plastic and Hand Surgery, University of Erlangen Medical Center, ²Department of Plastic, Hand, and Microsurgery, Sana Hospital Hof, ³Department of Materials Science and Engineering, Friedrich-Alexander-University, Erlangen-Nürnberg, ⁴Department of Plastic Surgery, Bayreuth Municipal Hospital, ⁵Institute of Anatomy, Chair II, Friedrich Alexander University Erlangen-Nuremberg

The present protocol illustrates a method for assessing the biophysical properties of tendon repairs ex vivo. A polytetrafluoroethylene (PTFE) suture material was evaluated by this method and compared to other materials under different conditions.