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Swiss Tropical and Public Health Institute

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children
Peiling Yap 1,2, Thomas Fürst 1,2, Ivan Müller 1,2, Susi Kriemler 1,2, Jürg Utzinger 1,2, Peter Steinmann 1,2
1Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, 2University of Basel, Basel, Switzerland

Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.

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Environment

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
Simon Blaser 1,2,3, Hanspeter Diem 4, Andreas von Felten 4, Morgan Gueuning 1, Michael Andreou 5, Neil Boonham 6,7, Jennifer Tomlinson 6, Pie Müller 2,3, Jürg Utzinger 2,3, Beatrice Frey 1, Jürg E. Frey 1, Andreas Bühlmann 8
1Department of Method Development and Analytics, Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope

This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.

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Immunology and Infection

Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Stephanie Mauti 1, Monique Léchenne 2, Service Naïssengar 3, Abdallah Traoré 4, Vessaly Kallo 5,6, Casimir Kouakou 7, Emmanuel Couacy-Hymann 7, Morgane Gourlaouen 8, Céline Mbilo 9,10, Pati Patient Pyana 11, Enos Madaye 3, Ibrahima Dicko 4, Pascal Cozette 1, Paola De Benedictis 8, Hervé Bourhy 1, Jakob Zinsstag 9,10, Laurent Dacheux 1
1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 2Environment and Sustainability Institute, University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5Direction des Services Vétérinaires, 6Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, 7Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, 8FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, 9Swiss Tropical and Public Health Institute, 10University of Basel, 11Institut National de Recherche Biomédicale

We present a complete protocol for postmortem diagnosis of animal rabies under field conditions using a rapid immunochromatographic diagnostic test (RIDT), from brain biopsy sampling to final interpretation. We also describe further applications using the device for molecular analysis and viral genotyping.

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