Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP TagVenkatasalam Shanmugabalaji 1, Véronique Douet 1, Birgit Agne 2, Felix Kessler 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg
We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.