JoVE Logo

Sign In

Martin-Luther-Universitat Halle-Wittenberg

1 ARTICLES PUBLISHED IN JoVE

image

JoVE Journal

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Venkatasalam Shanmugabalaji 1, Véronique Douet 1, Birgit Agne 2, Felix Kessler 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved