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Kobe University Graduate School of Medicine

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Sota Iwatani 1, Makiko Yoshida 2, Keiji Yamana 1, Daisuke Kurokawa 1, Jumpei Kuroda 1, Khin Kyae Mon Thwin 1, Suguru Uemura 1, Satoru Takafuji 1, Nanako Nino 1, Tsubasa Koda, Masami Mizobuchi 4, Masahiro Nishiyama 1, Kazumichi Fujioka 1, Hiroaki Nagase 1, Ichiro Morioka 5, Kazumoto Iijima 1, Noriyuki Nishimura 1
1Department of Pediatrics, Kobe University Graduate School of Medicine, 2Department of Pathology, Kobe Children's Hospital, 3Department of Pediatrics, Hyogo College of Medicine, 4Department of Developmental Pediatrics, Shizuoka Children's Hospital, 5Department of Pediatrics, Nihon University School of Medicine

Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

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Biology

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
Katsuyuki Nishi 1,2, Akiomi Nagasaka 1,2, Taro Sakamaki 1,2, Kay Sadaoka 1,2, Masanori Miyanishi 1,2
1Hematopoietic Stem Cell Biology and Medical Innovation (HSCBMI), Department of Pediatrics, Kobe University Graduate School of Medicine, 2RIKEN Center for Biosystems Dynamics Research

We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.

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