Sanford Consortium for Regenerative Medicine

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery, Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

Biology

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

Neuroscience

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Barbara Calabrese^1,2, Regina M. Powers^1,2, Alexandria J. Slepian^1,2, Shelley Halpain^1,2

¹Division of Biological Sciences, University of California, San Diego, ²Sanford Consortium for Regenerative Medicine

This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.

Developmental Biology

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Sandra L. Leibel^1,2,3, Rachael N. McVicar^2,3, Alicia M. Winquist^2,3, Evan Y. Snyder^1,2,3

¹Department of Pediatrics, University of California, San Diego School of Medicine, ²Sanford Consortium for Regenerative Medicine, ³Sanford Burnham Prebys Medical Discovery Institute

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.