Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal EpitheliumJulie G. In *1, Jennifer Foulke-Abel *2, Elizabeth Clarke 1, Olga Kovbasnjuk 1
1Department of Internal Medicine, University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine
Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.