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Shanghai Institute of Pollution Control and Ecological Security

5 ARTICLES PUBLISHED IN JoVE

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Biology

Electron Cryotomography of Bacterial Cells
Songye Chen 1, Alasdair McDowall 1,2, Megan J. Dobro 1, Ariane Briegel 1,2, Mark Ladinsky 1,2, Jian Shi 2, Elitza I. Tocheva 1, Morgan Beeby 1,2, Martin Pilhofer 1,2, H. Jane Ding 1, Zhuo Li 1,2, Lu Gan 1, Dylan M. Morris 1, Grant J. Jensen 1,2
1Division of Biology, California Institute of Technology - Caltech, 2Howard Hughes Medical Institute, California Institute of Technology - Caltech

We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

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Environment

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
Zhuo Li 1,2, Fangting Ai 3, Jing Zhang 4, Zhenyang Yu 1,2, Daqiang Yin 1,2
1College of Environmental Sciences and Engineering, Tongji University, 2Shanghai Institute of Pollution Control and Ecological Security, 3Jiaxing Tongji Institute of Environment, 4College of Ecological Technique and Engineering, Shanghai Institute of Technology

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

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Cancer Research

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
Zhuo Li *1, Xiao Yang *1, Yilu Zhang 1, Xiaolong Yang 1, Xinxin Cui 1, Yanjuan Zhang 1, Wenfeng Gong 1, Huichen Bai 1, Ning Liu 1, Zhiyu Tang 1, Mingming Guo 1, Kang Li 1, Tong Zhang 1, Lai Wang 1, Xiaomin Song 1
1BeiGene (Beijing) Co., Ltd.

We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.

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Environment

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration
Fuqiang Liu 1, Fang Li 1,2, Chensi Shen 1,2, Zhiwei Wang 2,3, Wolfgang Sand 1,4, Yanbiao Liu 1,2
1Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, College of Environmental Science and Engineering, Donghua University, 2Shanghai Institute of Pollution Control and Ecological Security, 3State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, 4Institute of Biosciences, Freiberg University of Mining and Technology

A protocol for the rational design of a dual-functional electroactive filter consisting of carbon nanotubes and titanate nanowires is reported and their environmental applications towards Sb(III) oxidation and sequestration is presented.

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Environment

Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae
Bin Zhang 1,2, Xinyue Yang 2, Jing Zhao 3, Ting Xu 2,4, Daqiang Yin 2,4
1State Key Laboratory of Marine Geology, Tongji University, 2Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 3Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of 10 Shanghai Polytechnic University, 4Shanghai Institute of Pollution Control and Ecological Security

A detailed experimental protocol is presented in this paper for the evaluation of neurobehavioral toxicity of environmental pollutants using a zebrafish larvae model, including the exposure process and tests for neurobehavioral indicators.

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