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G.EN.E.R.A. Centers for Reproductive Medicine

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Human Blastocyst Biopsy and Vitrification
Roberta Maggiulli 1, Adriano Giancani 1,2, Danilo Cimadomo 1, Filippo M. Ubaldi 1, Laura Rienzi 1
1G.EN.E.R.A. Centers for Reproductive Medicine, 2DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

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Medicine

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
Roberta Maggiulli 1, Alberto Vaiarelli 1, Danilo Cimadomo 1, Adriano Giancani 1, Luisa Tacconi 1, Gemma Fabozzi 1, Filippo Maria Ubaldi 1, Laura Rienzi 1
1Clinica Valle Giulia, GeneraLife IVF

Oocyte cryopreservation is recognized by several international scientific societies as the gold standard for fertility preservation in postpubertal women. Appropriate clinical and laboratory strategies ensure maximum efficacy, efficiency, and safety of fertility preservation treatments.

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