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Center for iPS cell research and application (CiRA)

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
Ryo Ohta *1, Ryohichi Sugimura *1, Akira Niwa 1, Megumu K. Saito 1
1Center for iPS cell research and application (CiRA)

Here, we present a protocol to precisely form hemogenic endothelium from human pluripotent stem cells in a feeder- and xeno-free condition. This method will have broad applications in disease modeling and screening for therapeutic compounds.

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Developmental Biology

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
Chuang-Yu Lin 1, Michiko Yoshida 1,2, Li-Tzu Li 3, Megumu K. Saito 1
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

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