JoVE Logo

Sign In

VA Nebraska Iowa Health Care System

1 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Kristi J. Warren 1,3, Todd A. Wyatt 2,3,4
1Department of Internal Medicine, University of Utah, 2Research Service, VA Nebraska Iowa Health Care System, 3Department of Internal Medicine, University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health, University of Nebraska Medical Center

In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved