CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Fernando Lopitz-Otsoa¹, Teresa C Delgado¹, Sofía Lachiondo-Ortega¹, Mikel Azkargorta², Felix Elortza², Manuel S Rodríguez³, María Luz Martínez-Chantar¹

¹Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), ²Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, ³Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).