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Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Ileectomy-induced Bile Overaccumulation in Mouse Intestine
Rongli Zhang *1,2,3, Jonathan W. Ray 1,2, Mukesh K. Jain 1,2, Shuxin Han *1,2
1Department of Medicine, Case Cardiovascular Research Institute, Case Western Reserve University, 2Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, 3Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine, University Hospitals Cleveland Medical Center

Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.

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Bioengineering

Optical Imaging of Isolated Murine Ventricular Myocytes
Shuxin Han 1,2, Matt Klos 3, Sherry Morgenstern 3, Ramiz Ahmad 3, Isabella Pua 3, Shreyas Suresh 1, Kayla Hicks 3, Eric Devaney 3
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center

We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.

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