Janssen Research & Development, Inc.

2 ARTICLES PUBLISHED IN JoVE

Medicine

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

Ioanna Kosmidou¹, Shannnon Wooden¹, Brian Jones², Thomas Deering¹, Andrew Wickliffe¹, Dan Dan¹

¹Department of Cardiac Electrophysiology, Piedmont Heart Institute, ²Cardiac Rhythm, Medtronic Inc.

Effective pulmonary vein isolation utilizing a cryoballoon depends on complete pulmonary vein occlusion. The point of occlusion can be effectively predicted by direct analysis of pulmonary vein pressure waveform analysis during balloon inflation using a simple and reproducible technique.

Immunology and Infection

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Brian Jones¹, Shannon Hitchcock¹, Matthew Loza¹, Ravi Malaviya¹, Tatiana Ort¹, Fang Shen¹

¹Immunology, Janssen Research & Development, Inc.

A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.