Instituto de Neurobiología, UNAM

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Daniela Avila-González¹, Guadalupe García-López¹, Néstor E. Díaz-Martínez², Héctor Flores-Herrera³, Anayansi Molina-Hernández¹, Wendy Portillo⁴, Néstor F. Díaz¹

¹Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, ²Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), ³Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, ⁴Instituto de Neurobiología, UNAM

This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

Neuroscience

Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles

Daniela Ávila-González¹, Larry J. Young², Francisco Camacho¹, Raúl G. Paredes^1,3, Néstor F. Díaz⁴, Wendy Portillo¹

¹Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, ²Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, ³Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, ⁴Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología

We established the conditions to culture neural progenitor cells from the subventricular zone and dentate gyrus of the adult brain of prairie voles, as a complementary in vitro study, to analyze the sex-dependent differences between neurogenic niches that could be part of functional plastic changes associated with social behaviors.