Pfizer Worldwide Research and Development

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Shuang Chen¹, Andreas Giannakou¹, Jonathon Golas¹, Kenneth G. Geles¹

¹Oncology R&D group, Pfizer Worldwide Research and Development

An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.

Medicine

Application of Straight-needle, Three-tailed, Knot-free, Peritoneal Sutures in Laparoscopic Transabdominal Preperitoneal Hernia Repair

Ning Ma^*1, Zhen Zong^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Jia-lin Chen¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, ²Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Nanchang University

Here, we present a protocol to introduce a new peritoneal suture method. This method is called straight-needle, three-tailed, knot-free suture, and we will outline the manufacturing method and clinical application of this suture in detail.

Medicine

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Ning Ma^*1, Dai Tang^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Chuang-xiong Liu¹, Hao-nan Huang¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, ²Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi)

Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain.