JoVE Logo

Sign In

The University of Tennessee HSC

1 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Alexandria Slayden 1, Kelsey North 1, Shivantika Bisen 1, Alex M. Dopico 1, Anna N. Bukiya 1, Avia Rosenhouse-Dantsker 2
1Department of Pharmacology, Addiction Science and Toxicology, The University of Tennessee HSC, 2Department of Chemistry, University of Illinois at Chicago

Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved