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GH Pitié Salpêtrière

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Melina Thetiot 1, Rémi Ronzano 1, Marie-Stéphane Aigrot 1, Catherine Lubetzki 1, Anne Desmazières 1
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.

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Neuroscience

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
Elisa Mazuir 1, Anne-Laure Dubessy 1,2, Loane Wallon 1,3, Marie-Stéphane Aigrot 1, Catherine Lubetzki 1,2, Nathalie Sol-Foulon 1
1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

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