Traction Force Microscopy to Study B Lymphocyte ActivationAnita Kumari 1, Judith Pineau 1, Ana-Maria Lennon-Duménil 1, Martial Balland 2, Paolo Pierobon 1
1Institut Curie, PSL Research University, INSERM U932, 2Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)
Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.