McGovern Medical School at UT Health

2 ARTICLES PUBLISHED IN JoVE

Biology

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

¹Department of Neuroscience and Cell Biology, University of Texas Medical Branch

This protocol describes a method for real-time measurement of mitochondrial calcium fluxes by fluorescent imaging. The method takes advantage of a circularly permutated YFP-based dual-excitation ratiometric calcium sensor (ratiometric pericam-mt) selectively expressed in mitochondria.

Biochemistry

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Ritika Tewari^*1, Savannah J. West^*1,2, Bieerkehazi Shayahati¹, Askar M. Akimzhanov¹

¹Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, ²MD Anderson UT Health Graduate School

Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.