UC Davis School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

¹Department of Pharmacology, University of California, Davis

We demonstrate the use of DNA microarrays for expression profiling of the nervous system. We describe RNA quality control, sample labeling, and array hybridization and scanning.

Medicine

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Angel Alvarez¹, Gustavo A. Barisone¹, Elva Diaz¹

¹Department of Pharmacology, University of California, Davis

The Focus Formation Assay provides a straightforward method to assess the transforming potential of a candidate oncogene.

Biochemistry

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

David J. Speca¹, Elva Diaz¹

¹Department of Pharmacology, UC Davis School of Medicine

Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in mouse brain lysates.