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Deutsches Zentrum für Herz-Kreislaufforschung (DZHK)

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Genetics

Optimized High Quality DNA Extraction from Formalin-Fixed Paraffin-Embedded Human Atherosclerotic Lesions
Sabine Bauer 1,2,4, Jessica Pauli 1,2, Jaroslav Pelisek 1,3
1Department for Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technical University Munich, 2partner site Munich Heart Alliance, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), 3Department of Vascular Surgery, University Hospital Zurich, 4Department of Experimental Cardiology, German Heart Centre, Technical University Munich

Here, we present a protocol for semi-automated DNA extraction from formalin-fixed paraffin-embedded lesions of human carotid arteries. The tissue lysis is performed without toxic xylene, which is followed by an automated DNA extraction protocol, including a second lysis step, binding of DNA to paramagnetic particles for cellulose based binding, washing steps, and DNA elution.

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