Huazhong University of Science and Technology, Wuhan 430000,China

11 ARTICLES PUBLISHED IN JoVE

Biology

Drosophila Pupal Abdomen Immunohistochemistry

¹Department of Biological Sciences, University of Alabama

Antibody staining of the Drosophila pupae can enhance genetic analyses of adult abdominal developmental genetics. We present our protocol for dissection, fixation and antibody staining of staged Drosophila pupal abdomen.

Medicine

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

Lifeng Zhang^*1, Andree Lapierre^*1, Brittany Roy¹, Maili Lim¹, Jennifer Zhu¹, Wei Wang¹, Stephen B. Sampson¹, Kyuson Yun¹, Bonnie Lyons¹, Yun Li¹, Da-Ting Lin¹

¹The Jackson Laboratory

By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.

Neuroscience

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Yun Li¹, Brittany D. Roy¹, Wei Wang¹, Lifeng Zhang¹, Stephen B. Sampson¹, Da-Ting Lin¹

¹The Jackson Laboratory

By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.

Chemistry

Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization

Wei Wang¹, John M. Lester¹, Anthony E. Amorosa², Deborah L. Chance³, Valeri V. Mossine¹, Thomas P. Mawhinney^1,4

¹Department of Biochemistry, University of Missouri, ²Department of Biological Sciences, University of Missouri, ³Department of Molecular Microbiology & Immunology, University of Missouri, ⁴Department of Child Health, University of Missouri

An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.

Medicine

A Metadata Extraction Approach for Clinical Case Reports to Enable Advanced Understanding of Biomedical Concepts

John Harry Caufield^1,2, David A. Liem^1,2,3, Anders O. Garlid^1,2, Yijiang Zhou⁴, Karol Watson^1,3, Alex A. T. Bui^1,5,6,7, Wei Wang^1,7,8,9, Peipei Ping^1,2,3,7,8

We present a protocol and associated metadata template for the extraction of text describing biomedical concepts in clinical case reports. The structured text values produced through this protocol can support deep analysis of thousands of clinical narratives.

Medicine

Cloud-Based Phrase Mining and Analysis of User-Defined Phrase-Category Association in Biomedical Publications

Dibakar Sigdel^*1,2, Vincent Kyi^*1,2, Aiden Zhang^*1, Shaun P. Setty³, David A. Liem^1,2,4, Yu Shi⁵, Xuan Wang⁵, Jiaming Shen⁵, Wei Wang^1,6,7, JiaWei Han⁵, Peipei Ping^1,2,4,6

¹The NIH BD2K Center of Excellence in Biomedical Computing, University of California, Los Angeles, ²Department of Physiology, University of California, Los Angeles, ³Department of Pediatric and Adult Congenital Heart Surgery, Miller Children's and Women's Hospital and Long Beach Memorial Hospital, ⁴Department of Medicine/Cardiology, University of California, Los Angeles, ⁵NIH BD2K Program Centers of Excellence for Big Data Computing -- KnowEng Center, Department of Computer Science, University of Illinois at Urbana-Champaign (UIUC), ⁶Scalable Analytics Institute (ScAi), University of California, Los Angeles, ⁷Department of Computer Science, University of California, Los Angeles

We present a protocol and associated programming code as well as metadata samples to support a cloud-based automated identification of phrases-category association representing unique concepts in user selected knowledge domain in biomedical literature. The phrase-category association quantified by this protocol can facilitate in depth analysis in the selected knowledge domain.

Medicine

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Ali R. Ahmadi¹, Le Qi¹, Kenichi Iwasaki¹, Wei Wang¹, Russell N. Wesson¹, Andrew M. Cameron¹, Zhaoli Sun¹

¹Department of Surgery, Johns Hopkins University School of Medicine

The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

Environment

A Uniaxial Compression Experiment with CO₂-Bearing Coal Using a Visualized and Constant-Volume Gas-Solid Coupling Test System

Weitao Hou¹, Hanpeng Wang¹, Wei Wang¹, Zhongzhong Liu¹, Qingchuan Li¹

¹Research Center of Geotechnical and Structural Engineering, Shandong University

This protocol demonstrates how to prepare a briquette sample and conduct a uniaxial compression experiment with a briquette in different CO₂ pressures using a visualized and constant-volume gas-solid coupling test system. It also aims to investigate changes in terms of coal’s physical and mechanical properties induced by CO₂ adsorption.

Medicine

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Di Liu^*1, Xizhu Xu^*2, Yuejin Li², Jie Zhang¹, Xiaoyu Zhang¹, Qihuan Li¹, Haifeng Hou², Dong Li², Wei Wang^1,2,3, Youxin Wang¹

¹Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, ²School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences, Edith Cowan University

Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

Behavior

A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

Yi Xie¹, Sai-Yue Deng¹, Si-Miao Chen¹, Xue-Jiao Chen¹, Wen-Wen Lai¹, Li-Fang Huang¹, Li Ba¹, Wei Wang^1,2, Feng-Fei Ding^1,3

¹Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China, ²Key Laboratory of Neurological Diseases of Chinese Ministry of Education, The School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000,China, ³Department of Pharmacology, School of Basic Medical Science; State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China

Presented here is a protocol for chronic sleep fragmentation (CSF) model achieved by an electrically controlled orbital rotor, which could induce confirmed cognitive deficit and anxiety-like behavior in young wild-type mice. This model can be applied to explore the pathogenesis of chronic sleep disturbance and related disorders.

Neuroscience

Establishment of a Rat Model of Superior Sagittal-Sinus Occlusion via a Thread-Embolism Method

Weiyan Jiang^*1, Congcong Jin^*2, Weiming Xu^*3, Yingxian Li⁴, Yinghong Lin⁵, Shengxiang Liang^6,7, Wei Wang⁸

¹Department of Clinical Laboratory, the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, ²Rehabilitation Department, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, ³Department of Neurosurgery, the Hospital of Changle, ⁴Pediatrics Ward 2, Shouguang Hospital of Traditional Chinese Medicine, ⁵Fujian University of Traditional Chinese Medicine, ⁶National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, ⁷Rehabilitation Industry Institute, Fujian University of Traditional Chinese Medicine, ⁸Department of Neurosurgery, the First Affiliated Hospital of Wenzhou Medical University

Here, we establish a novel Sprague-Dawley (SD) rat model of superior sagittal sinus (SSS) thrombosis via a thread-embolization method, and the stability and reliability of the model were verified.