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AbbVie, Cambridge Research Center

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
Rui Wang 1, Zi-tong Huang 3, Wen-kang Ren 1, Jiao Zhang 1, Yao Zhang 1, Bo Tan 3, Ping Huang 1,2, Hong-ying Cao 1,2
1School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 2Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, 3School of Basic Medical Sciences, Guangzhou University of Chinese Medicine

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.

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Immunology and Infection

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
Sean Graham 1, Lei Gao 1, Rui Wang 1
1AbbVie, Cambridge Research Center

Here, we describe the methodology to knock out a gene of interest in the immune system using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease (Cas9)-based technologies and the evaluation of these mice in a cluster of differentiation 40 (CD40) agonistic antibody-induced colitis model.

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