Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany

1 ARTICLES PUBLISHED IN JoVE

Genetics

Isolation of High Purity Tissues from Developing Barley Seeds

Martin Kovacik¹, Anna Nowicka^1,2, Ales Pecinka¹

¹Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, ²The Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology

Here we present a protocol for high purity manual isolation and quality control of embryo, endosperm and seed maternal tissues during entire barley seed development.