Sign In

Institute of Cellular Biology and Pathology "Nicolae Simionescu"

1 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

An Efficient Method for Adenovirus Production
Madalina Dumitrescu 1, Violeta Georgeta Trusca 1, Ioana Madalina Fenyo 1, Anca Violeta Gafencu 1
1Institute of Cellular Biology and Pathology "Nicolae Simionescu"

Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved