An Efficient Method for Adenovirus ProductionMadalina Dumitrescu 1, Violeta Georgeta Trusca 1, Ioana Madalina Fenyo 1, Anca Violeta Gafencu 1
1Institute of Cellular Biology and Pathology "Nicolae Simionescu"
Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.