4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski MicroscopyVictor Escrich 1, Begoña Ezcurra 1, Eva Gómez-Orte 1, Cristina Romero-Aranda 1, Antonio Miranda-Vizuete 2, Juan Cabello 1
1CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, 2IBIS (Instituto de Biomedicina de Sevilla), Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain
Here, we present a protocol for preparing and mounting Caenorhabditis elegans embryos, recording development under a 4D microscope and tracing cell lineage.