UK Centre for Ecology and Hydrology

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Sophie M. Briffa¹, Jo Sullivan², Agnieszka Siupa², Pauline Carnell-Morris², Michele Carboni², Kerstin Jurkschat³, Ruud J. B. Peters⁴, Carolin Schultz⁵, Kang Hee Seol⁶, Sook-Jin Kwon⁶, Sehee Park⁷, Tae Hyun Yoon^6,7, Colin Johnston³, Stephen Lofts⁸, Eugenia Valsami-Jones¹

¹School of Geography, Earth and Environmental Sciences, University of Birmingham, ²Malvern Panalytical, ³Department of Materials, University of Oxford, ⁴Wageningen Food Safety Research, ⁵UK Centre for Ecology & Hydrology, ⁶Institute for Next Generation Material Design, Hanyang University, ⁷Department of Chemistry, College of Natural Sciences, Hanyang University, ⁸UK Centre for Ecology & Hydrology

The protocol described here aims to measure the hydrodynamic diameter of spherical nanoparticles, more specifically gold nanoparticles, in aqueous media by means of Nanoparticle Tracking Analysis (NTA). The latter involves tracking the movement of particles due to Brownian motion and implementing the Stokes-Einstein equation to obtain the hydrodynamic diameter.

JoVE Core

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Ana C. Quevedo¹, Emily Guggenheim¹, Sophie M. Briffa¹, Jessica Adams^2,3, Stephen Lofts², Minjeong Kwak⁴, Tae Geol Lee⁴, Colin Johnston⁵, Stephan Wagner⁶, Timothy R. Holbrook⁶, Yves U. Hachenberger⁷, Jutta Tentschert⁷, Nicholas Davidson¹, Eugenia Valsami-Jones¹

¹School of Geography, Earth and Environmental Sciences, University of Birmingham, ²UK Centre for Ecology and Hydrology, ³Natural England, ⁴Center for Nanosafety Metrology, Korea Research Institute of Standards and Science (KRISS), ⁵Department of Materials, University of Oxford, ⁶Department of Analytical Chemistry, Helmholtz-Centre for Environmental Research, ⁷Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

This study presents the benchmarking results for an interlaboratory comparison (ILC) designed to test the standard operating procedure (SOP) developed for gold (Au) colloid dispersions characterized by ultraviolet-visible Spectroscopy (UV-Vis), amongst six partners from the H2020 ACEnano project for sample preparation, measurement, and analysis of the results.