International Centre for Diarrhoeal Disease Research, Bangladesh

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Akhirunnesa Mily^1,2, Sadaf Kalsum¹, Marco Giulio Loreti¹, Rokeya Sultana Rekha³, Jagadeeswara Rao Muvva¹, Magda Lourda^1,4, Susanna Brighenti¹

¹Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, ²Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, ³Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, ⁴Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

This protocol provides a method to study Mycobacterium tuberculosis infection in human M1- or M2-polarized macrophages based on differentiation of peripheral-blood-monocytes to macrophage-like cells that are infected with the GFP-labeled virulent strain H37Rv, and analyzed with flow cytometry using a 10-color panel including expression of selected M1/M2 markers.