University of Mogi das Cruzes

1 ARTICLES PUBLISHED IN JoVE

Biology

Three-Dimensional Adipocyte Culture as a Model to Study Cachexia-Induced White Adipose Tissue Remodeling

Miguel L Batista Jr.^1,2, Tova Meshulam^2,3, Kathleen Desevin², Nabil Rabhi², Stephen R. Farmer²

¹Department of Integrated Biotechnology, University of Mogi das Cruzes, ²Department of Biochemistry, Boston University School of Medicine, ³Obesity Research Center, Department of Medicine, Boston University School of Medicine

This protocol describes a three-dimensional (3D) magnetic printing culture system that permits dissection of white adipose tissue (WAT) remodeling induced by a conditioned medium from cancer cells. Using a 3D culture system of UCP1+ adipocytes that express a green fluorescent protein (GFP) allows the study of beige adipocytes contributing to adipose tissue remodeling.