First Affiliated Hospital of Xi’an Jiaotong University

8 ARTICLES PUBLISHED IN JoVE

Medicine

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Rui Liang^*1, Wei Dong^*1,3, Xiaopeng Shen¹, Xiaoping Peng^1,3, Angie G. Aceves^1,2, Yu Liu¹

¹Department of Biology and Biochemistry, University of Houston, ²Department of Economics, University of Houston, ³Department of Cardiology, The First Affiliated Hospital of Nanchang University

In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

Biology

Pancreatic Islet Embedding for Paraffin Sections

Yahui Kong¹, Pantea Ebrahimpour^1,2, Yu Liu³, Chaoxing Yang¹, Laura C. Alonso¹

¹Diabetes Center of Excellence, UMass Medical School, ²Department of Medicine, Saint Vincent Hospital, ³Department of Pathology, Morphology Core, UMass Medical School

Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.

Medicine

Traditional Trail Making Test Modified into Brand-new Assessment Tools: Digital and Walking Trail Making Test

Wei Wei^*1, Hóngyi Zhào^*1,2, Yu Liu², Yonghua Huang¹

¹Department of Neurology, The Seventh Medical Center of PLA Army General Hospital, ²Department of Neurology, NO 984 Hospital of PLA

Here, we present a protocol to show how to perform two types of cognitive assessment tools derived from the paper-pencil version of the Trail Making Test.

Medicine

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment

Hóngyi Zhào^*1,2, Yanhai Zhang^*3, Cuiqiao Xia¹, Yu Liu², Zhiyi Li², Yonghua Huang¹

¹Department of Neurology, Chinese PLA General Hospital, ²Department of Neurology, NO 984 Hospital of PLA, ³Department of Cadre Ward, Xijing 986 Hospital, Fourth Military Medical University

This study proposed a digital handwriting analysis of characters in individuals with mild cognitive impairment to find more information than is revealed by traditional pencil–paper handwriting analysis.

Cancer Research

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Lanbo Zhao^*1, Weichu Zhao^*2, Qi Wang^*1, Dongxin Liang¹, Yu Liu³, Guoxing Fu⁴, Lu Han¹, Yiran Wang¹, Chao Sun¹, Qing Wang¹, Qing Song¹, Qiling Li¹, Qinrui Lu¹

¹Department of Obstetrics and Gynecology, First Affiliated Hospital of Xi’an Jiaotong University, ²School of Social Sciences, University of Manchester, ³Department of Pathology, First Affiliated Hospital of Xi’an Jiaotong University, ⁴Omega Bioservices Inc

Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).

Medicine

Augmented Reality Navigation-Guided Core Decompression for Osteonecrosis of Femoral Head

Qi Wang^*1,3, Qiuyuan Wang^*2,3, Ran Ding³, Youjie Yao⁴, Junjun Pan⁵, Weiguo Wang³

¹China-Japan Friendship School of Clinical Medicine, Peking University, ²Graduate School of Beijing University of Chinese Medicine, ³Department of Orthopedic Surgery, Beijing Key Lab of Immunes-Mediated Inflammatory Disease, China-Japan Friendship Hospital, ⁴Beijing Normal University, ⁵State Key Laboratory of Virtual Reality Technology and Systems, Beihang University

Augmented reality technology was applied to core decompression for osteonecrosis of the femoral head to realize real-time visualization of this surgical procedure. This method can effectively improve the safety and precision of core decompression.

Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training

Xin Zhang^*1, Cuiqiao Xia^*2, Xiuli Zhao³, Yu Liu³, Hóngyi Zhào^2,3, Yonghua Huang²

¹Department of Sport, Inner Mongolia Medical University, ²Department of Neurology, The Seventh Medical Center of PLA General Hospital, ³Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.

Cancer Research

Surgical Technique for Superior Cervical Ganglionectomy in a Murine Model

Qi Wang¹, Chun-Hao Chen¹, Hongbo Xu², Sylvie Deborde^1,3, Richard J. Wong^1,3

¹Department of Surgery, Memorial Sloan Kettering Cancer Center, ²Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, ³David M. Rubenstein Center for Pancreatic Cancer Research, Memorial Sloan Kettering Cancer Center

The present protocol describes a mouse model of the ablation of adrenergic innervation by identifying and resecting the superior cervical ganglion.