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German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Implantation of Combined Telemetric ECG and Blood Pressure Transmitters to Determine Spontaneous Baroreflex Sensitivity in Conscious Mice
René D. Rötzer 1, Verena F. Brox 1, Konstantin Hennis 1, Stefan B. Thalhammer 1, Martin Biel 1,2, Christian Wahl-Schott 3, Stefanie Fenske 1,2
1Center for Integrated Protein Science (CIPS-M) and Center for Drug Research, Department of Pharmacy, Ludwig-Maximilians-Universität München, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, 3Hannover Medical School, Institute for Neurophysiology

The baroreflex is a heart-rate regulation mechanism by the autonomic nervous system in response to blood-pressure changes. We describe a surgical technique to implant telemetry transmitters for continuous and simultaneous measurement of electrocardiogram and blood pressure in mice. This can determine spontaneous baroreflex sensitivity, an important prognostic marker for cardiovascular disease.

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Biology

Three-Dimensional Imaging of Aortic Tissues in Atherosclerosis
Ting Sun 1,2,3, Mohammad R. Monjezi 2, Xu Xu 2, Changjun Yin 1,2,3,4, Andreas J.R. Habenicht 2,4, Sarajo K. Mohanta 2,4
1Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, 2Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University, 3Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, 4German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance

Here, we present optimized tissue-clearing protocols to image the murine aorta in three dimensions (3D). We delineate state-of-the-art procedures for immunostaining, optical clearing, and imaging with the intent to define the anatomical proximity of the peripheral nervous system with atherosclerotic plaques and the adventitia in atherosclerosis.

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